细胞像技活体H值子成内p双光术

发布时间：2025-05-06 00:01:04 来源：跃然纸上网 作者：知识

参考文献：J. Am. Chem. Soc. doi:10.1021/ja202902d (2011)

生物探索推荐英文摘要：

Nature News: A microscopist’s litmus test

Jun 23,细胞 2011 Vol.474

Intracellular pH levels can now be measured with a protein that glows red.

The red fluorescent protein has been dubbed pHRed by its designers, Gary Yellen and his colleagues at Harvard Medical School in Boston, Massachusetts. Light-detecting molecules of pHRed are preferentially excited by light at a wavelength of either 440 nanometres in basic conditions or 585 nanometres in acidic conditions, and the excitation ratio of the two gives a read-out of pH.

pHRed’s fluorescence lifetime — the time between excitation and emission — also responds to pH. The authors used this feature in two-photon microscopy to track second-by-second pH changes in living cells.

A red pH sensor leaves the often-used green fluorescence wavelength available for simultaneous study of other cellular properties, such as energy metabolism, during multicolour imaging.

Reference: J. Am. Chem. Soc. doi:10.1021/ja202902d (2011)

美国哈佛大学医学院神经生物学系Gary Yellen等研究者最近研发了基因编码的成像红色荧光蛋白质pHRed在活体细胞内成像pH值的技术。采用一般条件下采用440纳米激发光或者在酸性环境下采用585纳米激发光可以获得不同的技术荧光读数，

活体细胞内pH值双光子成像技术

细胞内PH值现在可以通过蛋白质的活体红色荧光成像来测量。

作者采用红色荧光探针，细胞根据两个激发光获得荧光读数的双光比例就可以读出细胞内PH值。

细胞内PH值现在可以通过蛋白质的成像红色荧光成像来测量。采用双光子显微镜可以一秒一秒地跟着活体细胞内pH值变化。技术

pHRed的活体荧光寿命（从光激发到荧光发射的时间）也跟pH值有关。

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